Development of a loop-mediated isothermal amplification assay for detection of Phytophthora sojae
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چکیده
منابع مشابه
development of a loop-mediated isothermal amplification assay for rapid detection of yersinia enterocolitica via targeting a conserved locus
background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...
متن کاملRapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملDevelopment of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses.
Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), caused by capripoxviruses (CaPVs), are economically important diseases of sheep, goats, and cattle, respectively. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of CaPVs. LAMP primers were designed to target a conserved gene encoding the poly(A) polymerase small subunit (VP...
متن کاملDevelopment and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus.
AIM The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP) targeting highly conserved genomic regions of Capripoxvirus (CaPVs) and its comparative evaluation with real-time polymerase chain reaction (PCR). MATERIAL AND METHODS Lyophilized vaccine strain of sheeppox virus (SPPV) was used for optimization of LAMP ass...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2012
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.2012.02619.x